| 质粒名称: | pVQ CMV NanoV1-2a-EGFP ferritin |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79649 |
| 存储实验室: | Jeffrey Friedman |
|
载体骨架基本信息 |
|
| 载体名称: | pVQAd-AscI-CMV-NPa |
| 载体出品公司: | Viraquest |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6217 bp |
| 完整质粒大小: | 11351 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，腺病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | transient receptor potential vanillin 1,TRPV1 |
| 物种来源: | R. norvegicus (rat) |
| 插入大小: | 2512 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | avrII（未销毁） |
| 3' 克隆位点: | EcoRI（克隆过程中销毁） |
| 5' 测序引物 | ggtctatataagcagagctg |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pVQ CMV NanoV1-2a-EGFP ferritin was a gift from Jeffrey Friedman (Addgene plasmid # 79649 ; http://n2t.net/addgene:79649 ; RRID:Addgene_79649) |
| 参考文献部分: | Bidirectional electromagnetic control of the hypothalamus regulates feeding and metabolism. Stanley SA, Kelly L, Latcha KN, Schmidt SF, Yu X, Nectow AR, Sauer J, Dyke JP, Dordick JS, Friedman JM. Nature. 2016 Mar 31;531(7596):647-50. doi: 10.1038/nature17183. Epub 2016 Mar 23. 10.1038/nature17183 PubMed 27007848 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pVQ CMV NanoV1-2a-EGFP ferritin 11340 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses camelid anti-GFP nanobody fused to TRPV1 and GFP-ferritin
chimera fusion protein.
ACCESSION .
VERSION .
KEYWORDS pVQ CMV NanoV1-2a-EGFP ferritin
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11340)
AUTHORS Stanley SA, Kelly L, Latcha KN, Schmidt SF, Yu X, Nectow AR, Sauer
J, Dyke JP, Dordick JS, Friedman JM
TITLE Bidirectional electromagnetic control of the hypothalamus regulates
feeding and metabolism.
JOURNAL Nature. 2016 Mar 31;531(7596):647-50. doi: 10.1038/nature17183. Epub
2016 Mar 23.
PUBMED 27007848
REFERENCE 2 (bases 1 to 11340)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11340
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 389..411
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
polyA_signal 554..688
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(591..610)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 645..664
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
CDS complement(1328..1351)
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/label=FLAG
/translation="DYKDDDDK"
RBS 1506..1514
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
CDS complement(1928..2644)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(1970..1991)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 2319..2338
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 2578..2599
/label=EGFP-N
/note="EGFP, reverse primer"
CDS complement(2660..2713)
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS complement(5258..5599)
/codon_start=1
/product="GFP-binding fragment of a single-chain camelid
antibody (Rothbauer et al., 2008)"
/label=GFP nanobody
/translation="VQLVESGGALVQPGGSLRLSCAASGFPVNRYSMRWYRQAPGKERE
WVAGMSSAGDRSSYEDSVKGRFTISRDDARNTVYLQMNSLKPEDTAVYYSNVNVGFEYW
GQGTQVTVSS"
regulatory 5605..5614
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind complement(5643..5667)
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind 5694..5727
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter complement(5775..5978)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(5804..5824)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 5979..6282
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
protein_bind complement(6372..6392)
/gene="mutant version of attB"
/label=attB4
/bound_moiety="BP Clonase(TM)"
/note="core recombination site for the Gateway(R) BP
reaction"
primer_bind complement(6418..6434)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6419..6435)
/label=pBluescriptKS
/note="For pBluescript vector"
misc_signal 6476..6626
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
repeat_region 6714..6816
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
primer_bind complement(6844..6862)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6930..7034
/gene="bla"
/label=AmpR promoter
CDS 7035..7895
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(7253..7272)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 8066..8654
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8555..8574
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ctgcaaaaca gatacaaaac tacataagac ccccacctta tatattcttt cccaccctta
61 agccacgccc acacatttca gtacctcaat ctgtatcttc atcgctagag ccaaactcag
121 cgcgggtgca ggccagcacc aagtgatcgg gcctcagctc ctcggtcaca tccagcatca
181 caggctggtt cctaatatgt ttaccgccac actcgcaggg tctgcacctg gtgcgggtct
241 catcgtacct cagcaccttc cagatctgcc accgcgggga ctagagtcga cctcatggct
301 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
361 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
421 tcatcaccga aacgcgcgag gcagccggat cataatcagc cataccacat ttgtagaggt
481 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
541 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
601 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
661 catcaatgta tcttatcatg tctggatctc cccgcggtgg cggccgctcg agtctagagg
721 ccggccaagc ttttactatt ccggactatt attaacgcgt gctctcatca ccgtgtccca
781 gggtgtgctt gtcaaagaga tattctgcca tgccagcttc aggggcaccc atcttgcgta
841 agttggtcac gtggtcaccc agttctttaa tggatttcac ctgttcactc agataatacg
901 tctcaatgaa gtcacataag tggggatcat tcttgtcagt agccagtttg tgcagttcca
961 gtagtgactg attcacactc ttttccaagt gcagtgcaca ctccattgca ttcagcccgc
1021 tctcccagtc atcacggtct ggtttcttta tatcctgcag gaagattcgg ccacctcgct
1081 ggttctgcag cttcatcagt ttctcggcat gctccctctc ctcatgagat tggtggagaa
1141 agtatttggc aaagttcttc agagccacat catctcggtc aaaataacaa gacatagaca
1201 gatagacgta ggaggcatac aactccaggt tgatctggcg gttgatggca gcctccgcgt
1261 cctggtggta gttctggcgc acttgcgagg gagacgcggt ggtcatcacg cgggagccgc
1321 cgccgccctt gtcgtcgtcg tccttgtagt cggagccgcc gccgccgcgg gcgtcgtgct
1381 tgagagtgag gcgctcaaag agatactcgc ccagagaccc ctggggcgcg ccagtctgcg
1441 ctggttgtgg ccccgccacc ctgcggaggt tggtcagatg gttgcccatc ttcttgatga
1501 gtttcacctc cttatccaga tagtggcttt ccaggaagtc acagagatga gggtccgcgc
1561 gggcagaacc cagggcatgc agatccaaga gggcctgatt caggttcttc tccatggcca
1621 aggcagcttc catggcctcc tgggttttac cccattcatc ttgagatggc ttctgcacat
1681 cctggaagag tgcacggccc ccgcgatcgt tctgaaactc gaggagacgc tccgcgccct
1741 cgcgcttctc ctcggccaat tcgcggaaga agtggcctac gccctccaga gccacgtcat
1801 cccgatcaaa aaagaagccc agagagaggt aggtgtagga ggcccgcagg tgcaagttga
1861 ccaggcggtt cacggcagct tccacctcgg tggaataatt ctgacgaatc tgagaggtca
1921 tgacgtcctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc acgaactcca
1981 gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac tgggtgctca
2041 ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc tgctggtagt
2101 ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga
2161 tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag ttgtactcca
2221 gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt
2281 tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct
2341 tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg aagaagtcgt
2401 gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc agggtggtca
2461 cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc agggtcagct
2521 tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt
2581 cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg cccttgctca
2641 ccatggtgct agcagatctt gggccaggat tctcctcgac gtcaccgcat gttagcagac
2701 ttcctctgcc ctccttctcc cctgggacca tggaatcctt gaaaacctca gcatcctctg
2761 gcttaaggga tcccgtataa tgcttcagtt gaacttcttc ctgctgggtg gcatgtctat
2821 ctcgagtgct tgcatccctc agaaggggaa ccagggcaaa gttcttccag tttctccctg
2881 aaactcggcc tgacctcagg gagaagctca gggtgcgctt gacgccctca cagttgcctg
2941 ggtcctcgtt gatgataccc acattggtgt tccaggtagt ccagtttacc tcgtccaccc
3001 tgaaacacca ccggtagtca tccttgccgt caggagtgaa ccccacctgc agcagcttgc
3061 cagagcggaa ggccttcctc atgcacttca ggaagctctt ctctgtatcc aggatggtga
3121 tggctctctg cagcttccag atgttcttgc tctcttgtgc aatcttgttg acggtctcac
3181 ccatgagagc aatgagcatg ttgagcagaa ggatgtaggt gagaatcaca taggccagta
3241 acaggatgat gaagacagcc ttgaagtcgt agttctcagt gaactccagg tcgcccatgc
3301 cgatggtgaa cttgaacagc tccagacatg tggaatacag gctgttgtaa gagttacctg
3361 gcttgcaggc agacccccgg cacttgtgtg gtgtggactc cataggcaga gagttattct
3421 tcccatcctc aatcagtgtc accacagctg tggaaaatcc aaacaagaac acgaggtaga
3481 cgaacataaa ccggcatagg tctctgagga tcatcttctc aatcatgaca gcatagatgc
3541 ccatctgctg gaatcctcgg gtatagtaga gcatgttggt ccagcccatg gccagggaga
3601 acaccatgga agccacatac tccttgcgtt ggctgaagta cagtaccaca gacaccagca
3661 tgaacagcga ctgtacaaag aaaagtatct cactgtagct gtccacaaac aaactcttga
3721 gggatggtcg cctctgcagg aaatattgaa tccctcggaa gaagaagtag actcctcctg
3781 acacagacaa gatctctccg gtgactcgga aatagtcccc aacggtgttt ttcagcttat
3841 aggggggcaa gccttccaca ggccgatagt aggcagccgc ggtgaagatg atcatataca
3901 agtagtagac gaagaagttg aagtagaaga tgcgcttgac aaatctgtcc cacttgtcct
3961 gtaggagtcg gttcaagggt tccacgagaa gcatgtcatg acggttaggg gtctcactgc
4021 tgctgtaagc gatcacctcc agaaccgagt tcttttcaca ggtgtcaatg caggacaggt
4081 cataaaggga ggagtacact ggcccatagg cccattcggt gaacttcctg gataggtgtc
4141 ggcactcggg ttcatggatc tccctctgga gaatgtaggc caagaccccg atcttcccac
4201 tgctagcagc cagagccagt ggcgtgagcc ccttcctgtt ggtgatctct tccagcttca
4261 gcgtggggtg gagtttggcc cccaggatca agatctcgtt gtacatgctt gtcacgaact
4321 tggtgttgtc aactgtgtta tctgccacct ccaccagggc atgaagcacc gtgttgccca
4381 ctgagtcccg ggcgctgatg tctgcaggct gccaggagtt ctgcagcagg aacttcacaa
4441 tggccagctg gttggtgcac gcagccaggg acaggggcag ctcaccaaag tagaagccag
4501 gcctcccttt ggttttcttg aagaagtccc cgttagccgc agcctggaca tctgctccat
4561 tctccaccaa gagggtcacc agcgtcatgt tccgccgttc aatggcaatg tgcagtgctg
4621 tctggccctt gtagtagctg tctgtgtagc tggcattgac aaactgcttc aggctgtctg
4681 tcttccgggc aacgtccagg agcagagcga tggtgtcatt ctgcccattg tgcagattga
4741 gcatggcttt tagcagacag gtctttcctg tctctgggtc tttgaactcg ctgtcagtca
4801 ggcgcttctt gctcctctgc aggaagggca gcaggctctc cagctcctgg cagttactct
4861 gagccacagc atcgaagatg ctcctgcgat catagagcct cgggggcttc tcaccagcgg
4921 agacggagtc ctgggatgac ggcctgacac tggcaggtcc atccccaggc ctctggatag
4981 ttagaacaga gctgacagtg atgatagggc aggaagccag cccgccttcc tcataagggc
5041 agtccagggg agaggcctcc tccgagtcac ccttcccaaa aagccgggta cgacccctgg
5101 tagtgaagat gtggggcttg actggaggtg gcttgcagtt agggtctctg tctggagggt
5161 ccaggcagga gttctcttgg ggtggggact cagactcctc tgagtctaag ctagcccgtt
5221 gttccatcct agggtagtct gggacgtcat atggatatga ggagacggtg acctgggtcc
5281 cctggcccca gtactcaaag cccacattga cattcgagta atacacggcg gtgtcctcag
5341 gtttcaggct gttcatttgc agatacaccg tattcctggc gtcgtctctg gagatggtga
5401 atcggccctt cacggagtct tcataacttg aacgatcacc agcactactc atacccgcga
5461 cccactcgcg ctccttccct ggagcctggc gataccacct catactatag cgattgacgg
5521 ggaatccaga ggctgcacag gagagtctca gagacccccc cggctgcacc aaggctcccc
5581 cagactccac cagctgcaca tcggccatgg tggcactagt ccggtgaagg gggcggccgc
5641 ggagcctgct tttttgtaca aacttgggtc gaattcgccc ttcgagggac ctaataactt
5701 cgtatagcat acattatacg aagttatatt aagggttccg gggtacgcta gcggatctga
5761 cggttcacta aaccagctct gcttatatag acctcccacc gtacacgcct accgcccatt
5821 tgcgtcaatg gggcggagtt gttacgacat tttggaaagt cccgttgatt ttggtgccaa
5881 aacaaactcc cattgacgtc aatggggtgg agacttggaa atccccgtga gtcaaaccgc
5941 tatccacgcc cattgatgta ctgccaaaac cgcatcacca tggtaatagc gatgactaat
6001 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
6061 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
6121 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
6181 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
6241 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
6301 ctatgaacta atgaccccgt aattgattac tattaatgaa ttccctggtg ctgcaagggc
6361 gaattcggag ccaacttttc tatacaaagt tgatatcact agcgttaacg tcgacatcga
6421 taccgtcgac ctcgagttta aacggcgcgc cggtaccgga tctccctaga caaatattac
6481 gcgctatgag taacacaaaa ttattcagat ttcacttcct cttattcagt tttcccgcga
6541 aaatggccaa atcttactcg gttacgccca aatttactac aacatccgcc taaaaccgcg
6601 cgaaaattgt cacttcctgt gtacaccggc gcacaccaaa aacgtcactt ttgccacatc
6661 cgtcgcttac atgtgttccg ccacacttgc aacatcacac ttccgccaca ctactacgtc
6721 acccgccccg ttcccacgcc ccgcgccacg tcacaaactc caccccctca ttatcatatt
6781 ggcttcaatc caaaataagg tatattattg atgatgctag cttaattaat tgaagacgaa
6841 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
6901 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
6961 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
7021 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
7081 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
7141 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
7201 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
7261 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
7321 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
7381 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
7441 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
7501 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
7561 gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac
7621 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
7681 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
7741 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
7801 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
7861 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
7921 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
7981 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
8041 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
8101 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
8161 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
8221 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
8281 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
8341 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
8401 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
8461 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
8521 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
8581 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
8641 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
8701 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg
8761 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg
8821 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct
8881 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca
8941 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct
9001 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga
9061 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt
9121 tgaaggctct caagggcatc ggtcgaggac aggcctctca agtctgtata cggggacacg
9181 gacagccttt tcgtcaccga gcgtggacac cggctcatgg aaaccagagg taagaaacgc
9241 atcaaaaagc atgggggaaa cctggttttt gaccccgaac ggccagagct cacctggctc
9301 gtggaatgcg agaccgtctg cggggcctgc ggcgcggatg cctactcccc ggaatcggta
9361 tttctcgcgc ccaagctcta cgccctcaaa agtctgcact gcccctcgtg cggcgcctcc
9421 tccaagggca agctgcgcgc caagggccac gccgcggagg ggctggacta tgacaccatg
9481 gtcaaatgct acctggccga cgcgcagggc gaagaccggc agcgcttcag caccagcagg
9541 accagcctca agcgcaccct ggccagcgcg cagcccggag cgcacccctt caccgtgacc
9601 cagactacgc tgacgaggac cctgcgcccg tggaaagaca tgaccctggc ccgtctggac
9661 gagcaccgac tactgccgta cagcgaaagc cgccccaacc cgcgaaacga ggagatatgc
9721 tggatcgaga tgccgtagag caggtgaccg agctgtggga ccgcctggaa ctgcttggtc
9781 aaacgctcaa aagcatgcct acggcggacg gtctcaaacc gttgaaaaac tttgcttcct
9841 tgcaagaact gctatcgctg ggcggcgagc gccttctggc ggatttggtc agggaaaaca
9901 tgcgagtcag ggacatgctt aacgaagtgg cccccctgct cagggatgac ggcagctgca
9961 gctctcttaa ctaccagttg cagccggtaa taggtgtgat ttacgggccc accggctgcg
10021 gtaagtcgca gctgctcagg aacctgcttt cttcccagct gatctcccct accccggaaa
10081 ccgttttctt catcgccccg caggtagaca tgatcccccc atctgaactc aaagcgtggg
10141 aaatgcaaat ctgtgagggt aactacgccc ctgggccgga tggaaccatt ataccgcagt
10201 ctggcaccct ccgcccgcgc tttgtaaaaa tggcctatga cgatctcatc ctggaacaca
10261 actatgacgt tagtgatccc agaaatatct tcgcccaggc cgccgcccgt gggcccattg
10321 ccatcattat ggacgaatgc atggaaaatc ttggaggtca caagggcgtc tccaagttct
10381 tccacgcatt tccttctaag ctacatgaca aatttcccaa gtgcaccgga tacactgtgc
10441 tggtggttct gcacaacatg aatccccgga gggatatggc tgggaacata gccaacctaa
10501 aaatacagtc caagatgcat ctcatatccc cacgtatgca cccatcccag cttaaccgct
10561 ttgtaaacac ttacaccaag ggcctgcccc tggcaatcag cttgctactg aaagacattt
10621 ttaggcacca cgcccagcgc tcctgctacg actggatcat ctacaacacc accccgcagc
10681 atgaagctct gcagtggtgc tacctccacc ccagagacgg gcttatgccc atgtatctga
10741 acatccagag tcacctttac cacgtcctgg aaaaaataca caggaccctc aacgaccgag
10801 accgctggtc ccgggcctac cgcgcgcgca aaacccctaa ataaagacag caagacactt
10861 gcttgatcca aatccaaaca gagtctggtt ttttatttat gttttaaacc gcattgggag
10921 gggaggaagc cttcagggca gaaacctgct ggcgcagatc caacagctgc tgagaaacga
10981 cattaagttc ccgggtcaaa gaatccaatt gtgccaaaag agccgtcaac ttgtcatcgc
11041 gggcggatga acgggaagct gcactgcttg caagcgggct caggaaagca aagtcagtca
11101 caatcccgcg ggcggtggct gcagcggctg aagcggcggc ggaggctgca gtctccaacg
11161 gcgttccaga cacggtctcg taggtcaagg tagtagagtt tgcgggcagg acggggcgac
11221 catcaatgct ggagcccatc acattctgac gcaccccggc ccatgggggc atgcgcgttg
11281 tcaaatatga gctcacaatg cttccatcaa acgagttggt gctcatggcg gcggcggctg
//
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